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You can not only play as Mario in Super Mario Run game. To make the game more exciting, you can unlock 5 more characters as you progress in the game. Though once you signed up in the game, Mario and Toad will be made available for you to play.
You need to do some tasks in order to unlock other characters. These Characters can also be used in different game modes such as Toad Rally. Each character has different play styles. Their unique abilities will help you progress in the game.
Here is some information about characters abilities including instructions on how to unlock each character. You need to play different modes in order to collect coins and toads.
Mario is the default playable character in the game. He grows bigger every time he grabs the power mushroom which his main power up. He can break bricks and kill enemies easily. He's always the on-the-go person you can count on.
Luigi is the twin brother of Mario. He's quite shy and easily gets scared (as you noticed in the previous Mario series) He can jump higher compared to Mario so it's easy for him to jump in higher gaps. You can unlock Luigi if you purchase the Luigi house in Kingdom Builder. But before you can purchase the Luigi house, you'll need to collect 150 green and purple toads and 1000 coins to build it. You can collect the green and purple toads in Toad Rally Mode.
Princess Peach is the beautiful Princess of Mushroom Kingdom. She can do floaty jumps to travel at a great distance. You need to complete all the 6 worlds with 24 levels in World Tour in order for you to unlock Peach.
Toad is the faithful servant of Princess Peach. He's the fastest among the characters in Mario world. This will be unlocked once you signed up and linked your My Nintendo account. It will serve as your reward.
Toadette is the referee in Toad Rally mode. Her pigtail mushroom head made her unique and the cutest in the Toad world. Just like Luigi, you need to build the Toadette's House. Just recruit 200 toads in each color (Red, Blue, and Green) in Toad Rally mode. Then the build cost 1000 coins.
As you play through each level, you'll meet some unique enemies. They are not part of the unlockable characters but plays a big role in the World Tour. Below is the list of names of the enemies you can encounter in the game.